Composite

Part:BBa_K1882001

Designed by: Milena Krach   Group: iGEM16_Tuebingen   (2016-10-14)


LDH Promotor-Phosphofructokinase

This part encodes BBa_K1882005 and BBa_K1882007 resulting in a L.johnsonii ATCC33200 optimized phosphofructokinase under the control of a constitutive LDH-promotor. This leads to overexpression of the phosphofructokinase in lactobacillus Johnsonii ATCC33200 when transferring this part into a lactobacillus expression vector.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 93
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None